Mi-crobiota dysbiosis occurs when energy imbalances appear due to an unhealthy diet and a sedentary lifestyle. The gut is a selective barrier that not only allows the translocation of nutrients from food, but also microbe-derived metabolites to the systemic circulation that flows through the liver. The technique reported here offers a valuable analytical tool for use in studies of SCFA production in the gut and their distribution to host tissues. The quantification accuracy ranged from 92% to 120%. The intra- and inter-day precisions in three levels of known concentrations were <12% and <20%, respectively. The calibration curves for all the analytes were linear with correlation coefficients r2 > 0.998. The limits of detection were all 0.001 mM except for acetate which was 0.003 mM. The technique is specific and sensitive for the quantification of acetate, butyrate, isobutyrate, isovalerate, lactate, propionate and valerate. The technique has significant advantages over the current widely used techniques based on sample derivatization and GC-MS analysis, including fast and simple sample preparation and short LC runtime (10 min). Here we present a novel, sensitive, and direct LC-MS/MS technique using isotopically labelled internal standards without derivatisation for the analysis of SCFAs in different biological matrices. Short chain fatty acids (SCFAs) are key metabolites produced by the gut microbiota from the fermentation of dietary fibre. The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases.
0 Comments
Leave a Reply. |